Caspase 9 (ICE-LAP6, Mch6, Apaf 3) | US Biological C2088-01D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Caspase 9 (ICE-LAP6, Mch6, Apaf 3)

catalog :

C2088-01D

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4i14(C9)

reactivity :

human

product information

Catalog Number :

C2088-01D

Product wo Prefix :

Caspase 9 (ICE-LAP6, Mch6, Apaf 3)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

4i14(C9)

Isotype :

IgG1

Desc1 :

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47kD procaspase-9/Apaf 1. This complex processes procaspase-9 into a large active fragment (35kD or 17kD) and a small fragment (10kD) by self-cleavage at Asp315 (3-5). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (6–9). In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at Asp330. This process serves as positive feedback to amplify the apoptotic signal in the caspase activation pathway (3–5). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hm Hu Mk Mo Rt

Immunogen :

Recombinant protein corresponding to human caspase-9.

Specificity :

Detects endogenous levels of the pro form of caspase-9 as well as cleaved fragments. Human: 47/37/35kD and mouse and rat: 51/39/37kD. Species Crossreactivity: Human, mouse, monkey, hamster and rat.

Purity :

Ascites

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 4i14(C9) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Ascites Immunogen: Recombinant protein corresponding to human caspase-9. Specificity: Detects endogenous levels of the pro form of caspase-9 as well as cleaved fragments. Human: 47/37/35kD and mouse and rat: 51/39/37kD. Species Crossreactivity: Human, mouse, monkey, hamster and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

(1) Duan, H. et al. (1996) J. Biol. Chem. 271, 16720–16724.(2) Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099–27106.(3) Liu, X. et al. (1996) Cell 86, 147–157.(4) Li, P. et al. (1997) Cell 91, 479–489.(5) Zou, H. et al. (1999) J. Biol. Chem. 274, 11549–11556.(6) Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215–2223.(7) Slee, E. A. et al. (1999) J. Cell Biol. 144, 281–292.(8) Sun, X. et al. (1999) J. Biol. Chem. 274, 5053–5060.(9) MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469–479.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/25/C2088-01D_1.jpg