Caspase 8, Cleavage Site (Asp384) (FLICE, MASH, Mch5) | US Biological C2087-48 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Caspase 8, Cleavage Site (Asp384) (FLICE, MASH, Mch5)

catalog :

C2087-48

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3A52(11G10)

reactivity :

human

product information

Catalog Number :

C2087-48

Product wo Prefix :

Caspase 8, Cleavage Site (Asp384) (FLICE, MASH, Mch5)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

3A52(11G10)

Isotype :

IgG1

Desc1 :

Apoptosis induced by CD95 (Fas/APO-1) and tumor necrosis factor receptor 1(TNFR1) activates caspase-8 (MACH/FLICE/Mch5), leading to the release of the active subunits p18 and p10 of caspase-8 (1-3). Activated caspase-8 is able to cleave and activate downstream caspases such as caspase-1, caspase-3, caspase-6 and caspase-7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to the N-terminal sequence of p10 of human caspase-8.

Specificity :

Recognizes endogenous levels of the small subunit of human caspase-8 resulting from cleavage at Asp384. Does not crossreact with full length caspase-8.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 3A52(11G10) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to the N-terminal sequence of p10 of human caspase-8. Specificity: Recognizes endogenous levels of the small subunit of human caspase-8 resulting from cleavage at Asp384. Does not crossreact with full length caspase-8. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Muzio, M., et al., Cell 85: 817-827 (1996). 2. Boldin, M.P., et al., Cell 85: 803-815 (1996). 3. Fernandes-Alnemri, T., et al., PNAS USA 93: 7464-7469 (1996).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/42/C2087-48_1.jpg