Caspase 6 (Mch2) | US Biological C2087-27A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Caspase 6 (Mch2)

catalog :

C2087-27A

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

C2087-27A

Product wo Prefix :

Caspase 6 (Mch2)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes. Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated. The activated upstream caspases further process downstream executioner caspases, such as caspase-3 and caspase-6, by cleaving them into large and small subunits, thereby initiating a caspase cascade leading to programmed cell death. One of the major targets for caspase-6 is the membrane associated protein lamin A. The cleavage of this protein causes cell membrane malfunction, membrane blebbing and eventual cell death. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding the cleavage site of caspase-6.

Specificity :

Recognizes endogenous levels of both full length caspase-6 (35kD) and the small subunit of caspase-6 resulting from cleavage at aspartic acid 193 (15kD). Does not recognize other caspases. Species Crossreactivity: human, mouse and rat.

Purity :

Purified by Protein A and and peptide affinity chromatography.

Form :

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

As reported

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: As reported Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding the cleavage site of caspase-6. Specificity: Recognizes endogenous levels of both full length caspase-6 (35kD) and the small subunit of caspase-6 resulting from cleavage at aspartic acid 193 (15kD). Does not recognize other caspases. Species Crossreactivity: human, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Cohen, G.M., Biochem. J. 326: 1–16 (1997). 2. Faleiro, L., et al., EMBO J. 16: 2271–2281 (1997). 3. Li, P., et al., Cell 91: 479–489 (1997). 4. Slee, E.A., et al., J. Cell Biol. 144: 281–292 (1999). 5. MacFarlane, M., et al., J. Cell Biol. 137: 469–479 (1997). 6. Orth, K., et al., J. Biol. Chem. 271: 16,443–16,446 (1996). 7. LeBlanc, A., et al., J. Biol. Chem. 274: 23,426–23,436 (1999).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/5/C2087-27A_1.jpg