antibody

Caspase 4 (Ich-2, ICErel-II, TX)

C2087-24A4

100 ul

polyclonal

domestic rabbit

nonconjugated

Caspase 4 (Ich-2, ICErel-II, TX)

rabbit

Pab

Antibodies

100 ul

IgG

Caspase-4 (TX/ICH-2/ICE relII) is a member of the caspase family of proteases that play a key role in the execution of apoptosis and activation of inflammatory cytokines (1-3). Expression of caspase-4 has been observed in most tissues except brain, with highest levels in placenta, lung, spleen, and peripheral blood lymphocytes (PBL). Caspase-4 was originally found to contribute to Fas-mediated apoptosis (4). Several caspases (including caspase-4, caspase-5, and mouse caspase-11 and -12) are most closely related to caspase-1 and are capable of inducing apoptosis when over-expressed but are better characterized in the proteolytic activation of inflammatory cytokines (5). Caspase-4 associates with TRAF6 and is involved in the LPS inducible production of inflammatory cytokines IL-8 and MIP1 in THP-1 cells (6). While caspase-4 and mouse caspase-12 localize to the endoplasmic reticulum (ER) and may be activated by drugs that induce ER-stress (7), at least one study suggests that caspase-4 and caspase-12 are not essential for the ER-stress induced apoptosis (8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic peptide corresponding to residues surrounding Ile125 within the p20 subunit of human caspase-4. Species sequence homology: monkey (100%)

Recognizes endogenous levels of total human caspase-4. Processing intermediate forms of caspase-4 are observed at 40kD and 32kD. Does not cross-react with other caspases.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Ile125 within the p20 subunit of human caspase-4. Species sequence homology: monkey (100%) Specificity: Recognizes endogenous levels of total human caspase-4. Processing intermediate forms of caspase-4 are observed at 40kD and 32kD. Does not cross-react with other caspases. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Faucheu, C. et al. (1995) EMBO J 14, 1914-22, 2. Kamens, J. et al. (1995) J Biol Chem 270, 15250-6, 3. Munday, N.A. et al. (1995) J Biol Chem 270, 15870-6, 4. Kamada, S. et al. (1997) Oncogene 15, 285-90, 5. Martinon, F. and Tschopp, J. (2007) Cell Death Differ 14, 10-22, 6. Lakshmanan, U. and Porter, A.G. (2007) J Immunol 179, 8480-90, 7. Hitomi, J. et al. (2004) J Cell Biol 165, 347-56, 8. Obeng, E.A. and Boise, L.H. (2005) J Biol Chem 280.

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