antibody

Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)

C2087-16B

100 ul

monoclonal

domestic rabbit

nonconjugated

9j115(5A1E)

Caspase 3, Cleaved (Asp175) (CPP-32, Apoptain, Yama, SCA-1)

rabbit

Mab

Antibodies

100 ul

9j115(5A1E)

IgG

Caspase-3 is one of the key executioners of apoptosis, being responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADPribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 subunits. Cleavage of caspase-3 requires aspartic acid at the P1 position. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:6400 Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Immunohistochemistry (Frozen): 1:800 using 3% formaldehyde as fixative. Immunohistochemistry (Paraffin): 1:1000 Immunofluorescence (IF-IC): 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic peptide corresponding to N-terminal residues adjacent to Asp175 in human Caspase 3. Species Sequence Homology: canine

Recognizes endogenous levels of the large fragment (17/19kD) of activated human Caspase 3 resulting from cleavage adjacent to Asp175. Does not recognize full length Caspase 3 or other cleaved caspases. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g. pancreatic alpha-cells). Cytplasmic background may be observed in human and monkey samples. Species Crossreactivity: mouse, rat and monkey

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not Determined

Product Type: Mab Isotype: IgG Clone No: 9j115(5A1E) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to N-terminal residues adjacent to Asp175 in human Caspase 3. Species Sequence Homology: canine Specificity: Recognizes endogenous levels of the large fragment (17/19kD) of activated human Caspase 3 resulting from cleavage adjacent to Asp175. Does not recognize full length Caspase 3 or other cleaved caspases. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g. pancreatic alpha-cells). Cytplasmic background may be observed in human and monkey samples. Species Crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IC IF IHC IP WB

-20°C

1. Alnemri, T. et al. (1994) J. Biol. Chem. 269, 30761–30764. 2. Nicholson, D. W. et al. (1995) Nature 376, 37–43.

https://usbio-images.r.worldssl.net/prodimages/49/C2087-16B_1.jpg

Immunohistochemical analysis of paraffin-embedded mouse embryo, using C2087-16B in the presence of control peptide (left) or Cleaved Caspase-3 Blocking Peptide (right).

https://usbio-images.r.worldssl.net/prodimages/49/C2087-16B_5.jpg

Flow Cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using C2087-16B compared to a nonspecific negative control antibody (red).