Caspase 3 (CPP-32, Apoptain, Yama, SCA-1) | US Biological C2087-16 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

catalog :

C2087-16

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

C2087-16

Product wo Prefix :

Caspase 3 (CPP-32, Apoptain, Yama, SCA-1)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Caspase-3 is one of the key executioners of apoptosis, being responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADPribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 subunits. Cleavage of caspase-3 requires aspartic acid at the P1 position (2). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:200 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding the cleavage site of human caspase-3.

Specificity :

Recognizes human caspase-3 (35kD) and the large fragment of caspase-3 resulting from cleavage (17, 19kD). Species Crossreactivity: mouse, rat and monkey.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding the cleavage site of human caspase-3. Specificity: Recognizes human caspase-3 (35kD) and the large fragment of caspase-3 resulting from cleavage (17, 19kD). Species Crossreactivity: mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Alnemri, T. et al. (1994) J. Biol. Chem. 269, 30761–30764. 2. Nicholson, D.W. et al. (1995) Nature 376, 37–43. 3. Erhardt, J.A. et al. (2001) Thromb. Res. 103, 143–148. 4. Hara, H. et al. (2002) J. Immunol. 168, 2288–2295. 5. Yu, L. et al. (2002) EMBO J. 21(14), 3749–3759.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/5/C2087-16_1.jpg

Picture4 Text :

Immunocytochemistry staining of NIH/3T3 cells (A, B) or HeLa cells (C, D), using C2087-16 (A, C) or the same antibody preincubated with caspase-3 peptide (B, D)