Caspase 3 (CPP-32, Apoptain, Yama, SCA-1) discontinued | US Biological C2087-03A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Caspase 3 (CPP-32, Apoptain, Yama, SCA-1) discontinued

catalog :

C2087-03A

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

9j114(8G10)

reactivity :

human

product information

Catalog Number :

C2087-03A

Product wo Prefix :

Caspase 3 (CPP-32, Apoptain, Yama, SCA-1) discontinued

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

9j114(8G10)

Isotype :

IgG

Desc1 :

Caspase-3 is one of the key executioners of apoptosis, being responsible either partially or totally for the proteolytic cleavage of many key proteins such as the nuclear enzyme poly (ADPribose) polymerase (PARP). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 subunits. Cleavage of caspase-3 requires aspartic acid at the P1 position. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% non-fat milk, 0.1% Tween-20 at 4°C with gentle shaking overnight. Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to amino-terminal residues adjacent to (Asp175) in human Caspase 3.

Specificity :

Recognizes endogenous levels of full length (35kD) and large fragment (17/19kD) of human Caspase 3 resulting from cleavage at (Asp175). Species Crossreactivity: mouse, rat and monkey

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 9j114(8G10) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to amino-terminal residues adjacent to (Asp175) in human Caspase 3. Specificity: Recognizes endogenous levels of full length (35kD) and large fragment (17/19kD) of human Caspase 3 resulting from cleavage at (Asp175). Species Crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Fernandes-Alnemri, T. et al. (1994) J. Biol. Chem. 269, 30761-30764. 2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/9/C2087-03A_1.jpg

Picture 6 File Name :

https://usbio-images.r.worldssl.net/prodimages/9/C2087-03A_6.jpg

Picture6 Text :

Immunoflourescence staining of caspase-3 in HeLa cells untreated (control ) or treated with staurosporine (apoptotic) using C2087-03A