Caspase 2 (ICH-1, Nedd2) | US Biological C2086-72D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Caspase 2 (ICH-1, Nedd2)

catalog :

C2086-72D

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4i13(C2)

reactivity :

human

product information

Catalog Number :

C2086-72D

Product wo Prefix :

Caspase 2 (ICH-1, Nedd2)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

4i13(C2)

Isotype :

IgG1

Desc1 :

Caspase-2 (Nedd2/ICH-1) is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved at Asp316, producing a 14kD fragment and a 32kD prodomain/large subunit. Subsequent processing at Asp152 and Asp330 produces an 18kD large subunit and a 12kD small fragment (1). Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD (2). This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events (3, 4). In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase- 3 activation, extending a possible role for caspase- 2 as a parallel effector caspase (5). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to the C-terminal portion of human caspase-2.

Specificity :

Recognizes endogenous levels of human procaspase-2 at ~48kD as well as its 14 and 12kD cleaved fragments.

Purity :

Ascites

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 4i13(C2) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic peptide corresponding to the C-terminal portion of human caspase-2. Specificity: Recognizes endogenous levels of human procaspase-2 at ~48kD as well as its 14 and 12kD cleaved fragments. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

(1) Li, H. et al. (1997) J. Biol. Chem 272, 21010–21017. 2) Tinel, A. and Tschopp, J. (2004) Science 304, 843–846. 3) Dirsch, V.M. et al. (2004) Oncogene 23, 1586–1593. 4) Castedo, M. et al. (2004) Oncogene (in press). 5) Paroni, G. et al. (2001) J. Biol. Chem. 276, 21907–21915.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/22/C2086-72D_1.jpg