antibody

Catenin, delta 1, phosphorylated (Tyr228) (CTNND1, Cadherin-Associated Protein delta 1, Cadherin-associated Src Substrate, CAS, p120 Catenin, p120(ctn), p120(cas), KIAA0384)

C2069-54M

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Catenin, delta 1, phosphorylated (Tyr228) (CTNND1, Cadherin-Associated Protein delta 1, Cadherin-associated Src Substrate, CAS, p120 Catenin, p120(ctn), p120(cas), KIAA0384)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

Catenin delta-1 (p120 catenin) has an amino- terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten, linked 42 amino-acid repeats. The carboxy-terminal tail has no known function (1). Catenin delta-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin delta-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin delta-1, which modulates RhoA, Rac and cdc42 proteins (1). Analogous to beta-catenin, catenin delta-1 translocates to the nucleus although its role at this location is unclear. Many studies show that catenin delta-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin delta-1 may function as a tumor suppressor (4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunofluorescence (IF-IC): 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic phosphopeptides corresponding to residues surrounding Tyr228 of human/mouse catenin delta-1.

Recognizes endogenous levels of human catenin delta-1 protein only when phosphorylated at Tyr228. Might cross react with another overexpressed phospho-tyrosine protein.

Purified by peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic phosphopeptides corresponding to residues surrounding Tyr228 of human/mouse catenin delta-1. Specificity: Recognizes endogenous levels of human catenin delta-1 protein only when phosphorylated at Tyr228. Might cross react with another overexpressed phospho-tyrosine protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF WB

-20°C

1. Reynolds, A.B. and Roczniak-Ferguson, A. (2004) Oncogene 23:7947–7956. 2. Davis, M. A. et al. (2003) J. Cell Biol. 163:525–534. 3. Thoreson, M.A. and Reynolds, A.B. (2002) Differentiation 70:583–589. 4. Anastasiadis, P.Z. and Reynolds, A.B. (2000) J. Cell Sci. 113:1319–1334. 5. Mariner, D.J. et al. (2001) J. Biol. Chem. 276:28006–28013. 6. Mariner, D.J. et al. (2004) J. Cell Sci. 117:1339–1350.

https://usbio-images.r.worldssl.net/prodimages/12/C2069-54M_1.jpg