Catenin, beta, phosphorylated (Ser45) | US Biological C2069-51 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Catenin, beta, phosphorylated (Ser45)

catalog :

C2069-51

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

C2069-51

Product wo Prefix :

Catenin, beta, phosphorylated (Ser45)

Host :

rabbit

Product Type :

Pab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1). It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3). GSK-3beta may destabilize beta-catenin by phosphorylating it at serines 33, 37 and threonine 41 (4). Serine 45 in beta-catenin is phosphorylated by CK1. This likely serves as a priming site for subsequent phosphorylation by GSK-3 (5-7). Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 (Mr 92kD) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser45 of human beta-catenin. Species sequence homology: mouse, rat, canine and porcine

Specificity :

Detects endogenous levels of betacatenin only when phosphorylated at serine 45. It does not recognize beta-catenin phosphorylated at other sites. Species Crossreactivity: Human

Purity :

Purified by Protein A and immunoaffinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and immunoaffinity chromatography. Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser45 of human beta-catenin. Species sequence homology: mouse, rat, canine and porcine Specificity: Detects endogenous levels of betacatenin only when phosphorylated at serine 45. It does not recognize beta-catenin phosphorylated at other sites. Species Crossreactivity: Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Cadigan, K.M. and Nusse, R., Genes Dev. 11: 3286-3305 (1997). 2. Wodarz, A. and Nusse, R., Annu. Rev. Cell Dev. Biol. 14: 59-88 (1998). 3. Polakis, P., Curr. Opin. Genet. Dev. 9: 15-21 (1999). 4. Yost, C., et al., Genes Dev. 10: 1443-1454 (1996). 5. Amit, S., et al., Genes Dev. 16: 1066-1076 (2002). 6. Lin, C., et al., Cell 108: 837-847 (2002). 7. Yanagawa, S., et al., EMBO J. 21: 1742 (2002). 8. Morin, P.J., Science 275: 1787-1790 (1997).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/45/C2069-51_1.jpg