Catenin, beta, non-phosphorylated (Ser33/37/Thr41) | US Biological C2069-50Q product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Catenin, beta, non-phosphorylated (Ser33/37/Thr41)

catalog :

C2069-50Q

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

product information

Catalog Number :

C2069-50Q

Product wo Prefix :

Catenin, beta, non-phosphorylated (Ser33/37/Thr41)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

B-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates B-catenin on Ser45. This phosphorylation event primes B-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3B destabilizes B-catenin by phosphorylating it at Ser33, Ser37 and Thr41 (7). Mutations in these phosphorylation sites, which result in the stabilization of B-catenin protein levels, have been found in many tumor cell lines (8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Ser37 of human beta-catenin. Species Sequence Homology: chicken, xenopus, zebrafish.

Specificity :

Recognizes endogenous levels of beta-catenin when residues Ser33, Ser37 and Thr41 are not phosphorylated. Species crossreactivity: mouse, rat and monkey

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Ser37 of human beta-catenin. Species Sequence Homology: chicken, xenopus, zebrafish. Specificity: Recognizes endogenous levels of beta-catenin when residues Ser33, Ser37 and Thr41 are not phosphorylated. Species crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305, 2. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88, 3. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21, 4. Amit, S. et al. (2002) Genes Dev. 16, 1066-1076, 5, Lin, C. et al. (2002) Cell 108, 837-847, 6. Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742, 7. Yost, C. et al. (1996) Genes Dev. 10, 1443-1454, 8. Morin, P.J. (1997) Science 275, 1787-1790, 9. Taurin, S. et al. (2006) J Biol Chem 281, 9971-6, 10. Hino, S. et al. (2005) Mol Cell Biol 25, 9063-72.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/16/C2069-50Q_1.jpg