antibody

Catenin, beta, phosphorylated (Ser675)

C2069-50F

20 ul

monoclonal

domestic rabbit

nonconjugated

phosphorylated

10E12(D2F1)

Catenin, beta, phosphorylated (Ser675)

rabbit

Mab

Phosphorylated

Antibodies

20 ul

10E12(D2F1)

IgG

B-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates B-catenin on Ser45. This phosphorylation event primes B-catenin for subsequent phosphorylation by GSK-3 (4-6). GSK-3B destabilizes B-catenin by phosphorylating it at Ser33, Ser37 and Thr41 (7). Mutations in these phosphorylation sites, which result in the stabilization of B-catenin protein levels, have been found in many tumor cell lines (8). PKA was shown to phosphorylate B-catenin at Ser675. Phosphorylation at Ser675 induces B-catenin accumulation in the nucleus and increases its transcriptional activity (9,10). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:200 Immunofluorescence (IF-IC): 1:100 Immunofluorescence (frozen): 1:100. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic phosphopeptide corresponding to residues surrounding Ser675 of human B-catenin. Species sequence homology: mouse, rat, chicken, xenopus and zebrafish (100%)

Recognizes endogenous levels of human beta-catenin only when phosphorylated at Ser675.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10E12(D2F1) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser675 of human B-catenin. Species sequence homology: mouse, rat, chicken, xenopus and zebrafish (100%) Specificity: Recognizes endogenous levels of human beta-catenin only when phosphorylated at Ser675. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IF IP WB

-20°C

1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305, 2. Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88, 3. Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21, 4. Amit, S. et al. (2002) Genes Dev. 16, 1066-1076, 5, Lin, C. et al. (2002) Cell 108, 837-847, 6. Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742, 7. Yost, C. et al. (1996) Genes Dev. 10, 1443-1454, 8. Morin, P.J. (1997) Science 275, 1787-1790, 9. Taurin, S. et al. (2006) J Biol Chem 281, 9971-6, 10. Hino, S. et al. (2005) Mol Cell Biol 25, 9063-72.

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