antibody

Calpain I, Large Subunit (Mu Calpain, Protein Kinase C Activating Factor)

C1035-26Y

100 ul

polyclonal

domestic rabbit

nonconjugated

Calpain I, Large Subunit (Mu Calpain, Protein Kinase C Activating Factor)

rabbit

Pab

Antibodies

100 ul

IgG

Calpain is a calcium-dependent thiol proteinase that is functionally active as a heterodimer composed of a regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). In vitro, Calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation. The regulation of calpain in vivo is the subject of many current studies, which suggest that proteolytic activity is regulated posttranscriptionally by mechanisms such as calcium requirements, subcellular localization of the heterodimer, phosphorylation via the EGFR-Erk signaling cascade, endogenous inhibitors (calpastatin) and autoproteolytic cleavage (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain between Gly27 and Leu28 (2). Calpain influences cell migration by modifying rather than degrading its substrates responsible for cell adhesion and cytoskeletal arrangement. Control of calpain activity has caught the attention of drug development since limiting its activity could mute invasiveness of tumors or chronic inflammation (1). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic peptide (KLH-coupled) derived from the human sequence of calpain 1 (large subunit).

Detects endogenous levels of total human calpain 1 (large subunit) protein. The antibody Detects full-length calpain 1 as well as calpain 1 autoproteolytically cleaved at leucine 28. Species Crossreactivity: mouse and rat

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide (KLH-coupled) derived from the human sequence of calpain 1 (large subunit). Specificity: Detects endogenous levels of total human calpain 1 (large subunit) protein. The antibody Detects full-length calpain 1 as well as calpain 1 autoproteolytically cleaved at leucine 28. Species Crossreactivity: mouse and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

(1) Perrin, B.J. and Huttenlocher, A. (2002) Int J Biochem Cell Biol. 34, 722–725. (2) Melloni, E. et al. (1996) Biochem Biophys Res Commun. 229, 193–197.

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