Calmodulin-Dependent Protein Kinase II (CAMKII) | US Biological C1035-25E product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Calmodulin-Dependent Protein Kinase II (CAMKII)

catalog :

C1035-25E

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

C1035-25E

Product wo Prefix :

Calmodulin-Dependent Protein Kinase II (CAMKII)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

CaMKII is an important member of calcium/ calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates itself at threonine 286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. On the other hand, PKA (protein kinase A) prevents this dephosphorylation process through its inhibitory effect on PP1 (4). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide around the N-terminal amino acid sequence of human CaMKII.

Specificity :

Recognizes endogenous levels of total human CaMKII. Species Crossreactivity: mouse, rat and monkey

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide around the N-terminal amino acid sequence of human CaMKII. Specificity: Recognizes endogenous levels of total human CaMKII. Species Crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

(1) Hughes, K. et al. (2001) J. Biol. Chem. 276, 36008–36013. (2) Barria, A. et al. (1997) Science 276, 2042–2045. (3) Means, A.R. (2000) Mol. Endocrinol. 14, 4–12. (4) Makhinson, M. et al. (1999) J. Neurosci. 19, 2500–2510.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/43/C1035-25E_1.jpg