Cadherin P (Cadherin 3, Placental, CDH3) | US Biological C0108-37B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Cadherin P (Cadherin 3, Placental, CDH3)

catalog :

C0108-37B

quantity :

20 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

8A8(C13F9)

reactivity :

human

product information

Catalog Number :

C0108-37B

Product wo Prefix :

Cadherin P (Cadherin 3, Placental, CDH3)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ul

Clone # USB :

8A8(C13F9)

Isotype :

IgG

Desc1 :

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, Band E-cadherins as well as about ten other members which are found in adherens junctions (AJ), a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with beta-catenin, gamma-catenin (also called plakoglobin) and p120 catenin. beta-catenin and gamma-catenin associate with alpha-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Unlike beta and gamma-catenin, p120 regulates cadherin adhesive activity and trafficking rather than having a structural role in the junctional complex (1-4). E-cadherin is considered an acting suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-Cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression and localization are correlated with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). Applications: Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to residues near the C-terminus of human P-cadherin. Species sequence homology: monkey (100%)

Specificity :

Recognizes endogenous levels of total human P-cadherin protein. This antibody does not cross-react with other cadherin family members.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 8A8(C13F9) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues near the C-terminus of human P-cadherin. Species sequence homology: monkey (100%) Specificity: Recognizes endogenous levels of total human P-cadherin protein. This antibody does not cross-react with other cadherin family members. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IF WB

Storage Temperature :

-20°C

Reference :

1. Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235. 2. Christofori, G. (2003) EMBO J. 22, 2318-2323. 3. Hazan, R.B. et al. (2004) Ann. NY Acad. Sci. 1014, 155-163. 4. Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol. 14, 427-434. 5. Rabascio, C. et al. (2004) Cancer Res. 64, 4373-4377. 6. Yamaoka-Tojo, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26, 1991-1997. 7. Patel, I.S. et al. (2003) Int. J. Cancer 106, 172-177. 8. Sanders, D.S. et al. (2000) J. Pathol. 190, 526-530.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/43/C0108-37B_1.jpg