antibody

ErbB3, phosphorylated (Tyr1222) (c-erbB3, HER3)

C0026-09D

100 ul

monoclonal

domestic rabbit

nonconjugated

phosphorylated

7H10(50C2)

ErbB3, phosphorylated (Tyr1222) (c-erbB3, HER3)

rabbit

Mab

Phosphorylated

Antibodies

100 ul

7H10(50C2)

IgG

HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase. There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, Grb2 and the p85 subunit of PI3 kinase, which mediate ErbB- downstream signaling. Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3 kinase. ErbB3 is highly expressed in many cancer cells and activation of ErbB3-PI3 kinase pathway is correlated with malignant phenotypes of adenocarcinomas. In tumor development, ErbB3 may function as an oncogenic unit together with other ErbB members, e.g. ErbB2 requires ErbB3 to drive breast tumor cell proliferation. Thus, prevention of the interaction of ErbB3 with other ErbB tyrosine kinases has become a novel anti-tumor strategy. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic phosphopeptide corresponding to residues surrounding Tyr1222 of human ErbB3.

Recognizes endogenous human ErbB3 only when phosphorylated at tyrosine 1222. Does not crossreact with other tyrosine phosphorylated receptor tyrosine kinases. Species Crossreactivity: mouse

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not Determined

Product Type: Mab Isotype: IgG Clone No: 7H10(50C2) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr1222 of human ErbB3. Specificity: Recognizes endogenous human ErbB3 only when phosphorylated at tyrosine 1222. Does not crossreact with other tyrosine phosphorylated receptor tyrosine kinases. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Yarden, Y. and Sliwkowski, M.X. Nature Rev. Mol. Cell. Biol. 2: 127–137 (2001). 2. Guy, P.M. et al. Proc. Natl. Acad. Sci. USA 91; 8132–8136 (1994). 3. Songyang, Z. et al. Cell 72: 767–778 (1993).4. Kim, H.H. et al. J. Biol. Chem. 269: 24747–24755 (1994) . 5. Sithanandam, G. et al. Carcinogenesis 24:1581–1592 (2003). 6. Kobayashi, M. et al. Oncogene 22: 1294–1301 (2003). 7. Holbro, T. et al. Proc. Natl. Acad. Sci. USA 100: 8933–8938 (2003).

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