antibody

c-Cbl, phosphorylated (Tyr731)

C0013-25

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

c-Cbl, phosphorylated (Tyr731)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

C-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells (1,2). c-Cbl is rapidly tyrosine phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol 3 kinase, Crk and 14-3-3 proteins (3,4). c-Cbl possesses a highly conserved N-terminal phosphotyrosine binding domain (TKB), a C3HC4 RING finger motif. TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. Its RING finger domain recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for c-Cbl proteins functioning as ubiquitin protein ligases and downregulators of RTKs (3). c-Cbl protein in human cancer tissues is frequently tyrosine phosphorylated in a tumor-specific manner (5). Phosphorylation of Tyr731 of c-Cbl provides a docking site for downstream signaling components such as p85 and Fyn (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic peptide (KLH coupled) corresponding to residues surrounding Tyr731 of human c-Cbl.

Detects overexpressed levels of Tyr731 phosphorylated c-Cbl. The antibody does not crossreact with other related tyrosine-phosphorylated proteins.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide (KLH coupled) corresponding to residues surrounding Tyr731 of human c-Cbl. Specificity: Detects overexpressed levels of Tyr731 phosphorylated c-Cbl. The antibody does not crossreact with other related tyrosine-phosphorylated proteins. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Blake, T.J. et al. (1991) Oncogene 6, 653–657. 2. Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473–482. 3. Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 294–307. 4. Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273, 8323–8331. 5. Kamei, T. et al. (2000) Int. J. Oncol. 17, 335–339. 6. Hunter, S. et al. (1999) J. Biol. Chem. 274, 2097–2106.

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