antibody

c-Abl, Phosphorylated (Tyr177) (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl)

C0013-06A

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

c-Abl, Phosphorylated (Tyr177) (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene (1). The N-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domain. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region may be involved in a GTPase-activating function for the small GTP-binding protein Rac (2,3). The function of Wild-type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstrean signal mediator (4). The Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) (5). Tyrosine 177 of Bcr is phosphorylated in the Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl (6). Phosphorylated Tyr177 of Bcr provides a docking site for Gab2 and GRB2 (7,8). Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:50 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Synthetic phosphopeptide corresponding to residues surrounding Tyr177 of human Bcr. Molecular Weight:160kD (Bcr), 210kD (Bcr-Abl).

ecognizes endogenous levels of human Bcr and Bcr-Abl only when phosphorylated at tyrosine 177. Species Crossreactivity: mouse

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr177 of human Bcr. Molecular Weight:160kD (Bcr), 210kD (Bcr-Abl). Specificity: ecognizes endogenous levels of human Bcr and Bcr-Abl only when phosphorylated at tyrosine 177. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IHC WB

-20°C

1. Groffen, J. et al. (1984) Cell 36, 93–99. 2. Maru, Y. et al. (1991) Cell 67, 459–468. 3. Che, W. et al. (2001) Circulation 104, 1399–1406. 4. Abe, J.I. et al. (2001) Ann. N.Y. Acad. Sci. 947, 341–343. 5. Voncken, J.W. et al. (1995) Cell 80, 719–728. 6. He, Y. et al. (2002) Blood 99, 2957–2968. 7. Sattler, M. et al. (2002) Cancer Cell 1, 479–492. 8. Warmuth, M. et al. (1995) J. Biol. Chem. 272, 33260–33270.

https://usbio-images.r.worldssl.net/prodimages/31/C0013-06A_1.jpg

Flow cytometric analysis of K562 cells, untreated (green) or STI571 treated (blue) using C0013-06A. Antibody compared to a nonspecific negative control antibody (red).