antibody

c-Abl, phosphorylated (Thr735) (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl)

C0013-06

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

c-Abl, phosphorylated (Thr735) (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl)

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response (1–3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyrosine 245 is located in the linker region between the SH2 and catalytic domains that is conserved among the Abl family. Phosphorylation of Tyr245 is involved in activation of c-Abl kinase activity (5). Tyrosine 412 is located in the kinase activation loop of c-Abl molecules; phosphorylation of this residue is required for the kinase activity (6). Thr735 is in a conserved 14-3-3 protein binding motif, which can be phosphorylated upon stress stimulation or TPA treatment (Wu, J. et al. unpublished data). Phosphorylation of Thr735 in c-Abl may play an important role in regulation of c-Abl localization as well as its function. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic phosphopeptide corresponding to residues surrounding Thr735 of human c-Abl.

Recognizes overexpressed levels of Thr735 phosphorylated c-Abl or Bcr-Abl. Does not crossreact with other related proteins.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined. Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr735 of human c-Abl. Specificity: Recognizes overexpressed levels of Thr735 phosphorylated c-Abl or Bcr-Abl. Does not crossreact with other related proteins. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650. 2. Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182. 3. Danial, N.N. et al. (2000) Oncogene 19, 2523-2531. 4. Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16. 5. Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637. 6. Pluk, H. et al. (2002) Cell 108, 247-259.

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