antibody

c-Abl, phosphorylated (Tyr89) (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl)

C0013-02C

100 ul

monoclonal

domestic rabbit

nonconjugated

phosphorylated

10g161(61A6)

c-Abl, phosphorylated (Tyr89) (ABL1, v-abl, Abelson Murine Leukemia Viral Oncogene Homolog 1, ABL, c-ABL, JTK7, p150, Proto-Oncogene Tyrosine-Protein Kanase ABL1, v-abl)

rabbit

Mab

Phosphorylated

Antibodies

100 ul

10g161(61A6)

IgG

The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6). Phosphorylation of c-Abl on Tyr89 was identified using LC-MS/MS platform for phosphorylation site discovery as well as another publication using MS technology (7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Synthetic phosphopeptide corresponding to residues surrounding Tyr89 of human c-Abl. Species sequence homology: mouse (100%)

Recognizes endogenous levels of human c-Abl only when phosphorylated at Tyr89. This antibody may cross-react with other tyrosine-phosphorylated proteins.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10g161(61A6) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr89 of human c-Abl. Species sequence homology: mouse (100%) Specificity: Recognizes endogenous levels of human c-Abl only when phosphorylated at Tyr89. This antibody may cross-react with other tyrosine-phosphorylated proteins. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650. 2. Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182. 3. Danial, N.N. et al. (2000) Oncogene 19, 2523-2531. 4. Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16. 5. Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637. 6. Pluk, H. et al. (2002) Cell 108, 247-259. 7. Meyn, M.A. et al. (2006) J. Biol. Chem. 281, 30907-30916.

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