antibody

Bcr (Breakpoint Cluster Region Protein, Renal Carcinoma Antigen NY-REN-26, BCR1, D22S11)

C0013-01C

100 ul

polyclonal

domestic rabbit

nonconjugated

Bcr (Breakpoint Cluster Region Protein, Renal Carcinoma Antigen NY-REN-26, BCR1, D22S11)

rabbit

Pab

Antibodies

100 ul

IgG

The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene (1). The N-terminal part of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domain. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The C-terminal part may be involved in a GTPase-activating function for the small GTP-binding protein Rac (2,3). The function of Wildtype Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstrean signal mediator (4). The Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) (5). Tyrosine 177 of Bcr is phosphorylated in Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl (6). Phosphorylated tyrosine 177 of Bcr provides a docking site for Gab2 and GRB2 (7,8). Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunocytochemistry: 1:100 Flow Cytometry: 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mo Rt

Synthetic peptide corresponding to the N-terminal sequence of human Bcr. MW of Antigen: 130, 160kD

Detects endogenous levels of total Bcr and the 210kD Bcr-Abl fusion protein. Species Crossreactivity: Human, mouse and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide (KLH coupled) corresponding to the N-terminal sequence of human Bcr. MW of Antigen: 130, 160kD Specificity: Detects endogenous levels of total Bcr and the 210kD Bcr-Abl fusion protein. Species Crossreactivity: Human, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IC WB

-20°C

(1) Groffen, J. et al. (1984) Cell 36, 93–99. (2) Maru, Y. et al. (1991) Cell 67, 459–468. (3) Che, W. et al. (2001) Circulation 104, 1399–1406. (4) Abe, J. I. et al. (2001) Ann. N.Y. Acad. Sci. 947, 341–343. (5) Voncken, J. W. et al. (1995) Cell 80, 719–728. (6) He, Y. et al. (2002) Blood 99, 2957–2968. (7) Sattler, M. et al. (2002) Cancer Cell 1, 479–492. (8) Warmuth, M. et al. (1995) J. Biol. Chem. 272, 33260–33270.

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