Bromodeoxyuridine (BrdU) discontinued | US Biological B2850-24B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU) discontinued

catalog :

B2850-24B

quantity :

250 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5G15

product information

Catalog Number :

B2850-24B

Product wo Prefix :

Bromodeoxyuridine (BrdU) discontinued

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

250 ul

Clone # USB :

5G15

Isotype :

IgG1

Desc1 :

BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. Applications: Immunohistology (Formalin/paraffin): (Use Ab at 1ug/ml for 30 min at RT) [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37°C] Positive Control: Cultured cells grown in presence of BrdU or tissues from experimental animals injected with BrdU. Cellular Localization: Nuclear Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

BrdU conjugated to BSA.

Specificity :

Recognizes Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. Species Crossreactivity: All species.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 5G15 Host: mouse Concentration: ~0.2mg/ml Form: Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide at 1mg/ml. Purity: Purified by Protein G affinity chromatography. Immunogen: BrdU conjugated to BSA. Specificity: Recognizes Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. Species Crossreactivity: All species. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC

Storage Temperature :

-20°C

Reference :

1. Magaud J P, et al. (1989) J Histochem Cytochem, 37:1517-1527.