Bromodeoxyuridine (BrdU) | US Biological B2850-20 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU)

catalog :

B2850-20

quantity :

4 x2.5 ml

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BU-1

product information

Catalog Number :

B2850-20

Product wo Prefix :

Bromodeoxyuridine (BrdU)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

4 x2.5 ml

Clone # USB :

BU-1

Isotype :

IgG2a

Desc1 :

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. Applications: Suitable for use in ELISA and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1:3 dilution of this lot showed positive immunostaining for BrdU in HeLa cells ﬁxed with 95% ethanol, 5% glacial acetic acid. Technical Tip: This antibody detects 5-Bromo-2- deoxyuridine incorporated into double-stranded DNA. DNA denaturation is not required. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 25% glycerol and store at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

5-iodouridine:ovalbumin conjugate.

Specificity :

Recognizes 5-Bromo-2-deoxyuridine.

Purity :

Supernatant.

Form :

Supplied as 4 vials. Each vial contains 2.5ul lyophilized mouse culture supernatant. Reconstitute each vial with 500ul of water containing 0.1% azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: BU-1 Host: mouse Concentration: Not determined Form: Supplied as 4 vials. Each vial contains 2.5ul lyophilized mouse culture supernatant. Reconstitute each vial with 500ul of water containing 0.1% azide. Purity: Supernatant. Immunogen: 5-iodouridine:ovalbumin conjugate. Specificity: Recognizes 5-Bromo-2-deoxyuridine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IC

Storage Temperature :

-20°C

Reference :

1. Gonchoroff, N.J., et. al., Cytometry; 6: 506–512, 1985. 2. Gonchoroff, N.J., et. al., J. Immunol. Methods; 93: 97–101, 1986. 3. Greipp, P.R., et. al., Mayo Clin. Proc; 62: 969–977, 1987. 4. Beauregard, P., et. al., Hematol Pathol; 5: 177–83, 1991.