Bromodeoxyuridine (BrdU) | US Biological B2850-15 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU)

catalog :

B2850-15

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BU-1

product information

Catalog Number :

B2850-15

Product wo Prefix :

Bromodeoxyuridine (BrdU)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

BU-1

Isotype :

IgG2a

Desc1 :

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Bromodeoxyuridine

Specificity :

Recognizes bromodeoxyuridine and crossreacts with iodouridine (10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.

Purity :

Culture supernatant.

Form :

Supplied as a liquid. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: BU-1 Host: mouse Concentration: Not determined Form: Supplied as a liquid. No preservative added. Purity: Culture supernatant. Immunogen: Bromodeoxyuridine Specificity: Recognizes bromodeoxyuridine and crossreacts with iodouridine (10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IC IHC

Storage Temperature :

-20°C

Reference :

Gonchoroff, NJ et al. A monoclonal antibody reactive with 5-Bromo-2- deoxyuridine that does not require DNA denaturation. Cytometry. 6:506–512, 1985. Greipp, PR et al. Immunofluorescent plasma cell labeling indices (LI) using a monoclonal antibody (BU-1). Am. J. Hematol. 20:289–292, 1985. Gonchoroff, NJ et al. S-phase detection with an antibody to bromodeoxyuridine. Role of Dnase pretreatment. J. Immunol. Methods. 97:97- 101, 1986. Greipp, PR et al. Immunofluorescent labeling indices in myeloma and related monoclonal gammopathies. Mayo Clin Proc. 62:969–977, 1987.