Bromodeoxyuridine (BrdU) (FITC) | US Biological B2850-10H product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU) (FITC)

catalog :

B2850-10H

quantity :

50 ug

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

Bu20a

product information

Catalog Number :

B2850-10H

Product wo Prefix :

Bromodeoxyuridine (BrdU) (FITC)

Host :

mouse

Product Type :

Mab

Conjugate :

FITC

Category :

Antibodies

Size1 :

50 ug

Clone # USB :

Bu20a

Isotype :

IgG1

Desc1 :

BrdU can be incorporated into DNA during S-phase of the cell cycle. The antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labelling techniques. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

Immunogen :

Bromodeoxyuridine (BSA).

Specificity :

Recognizes the thymidine analog bromodoxyuridine (BrdU), which can be incorporated into DNA during S-phase of the cell cycle.

Purity :

Purified by Protein G affinity chromatography from tissue culture supernatant.

Form :

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Concentration :

~0.1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: Bu20a Host: mouse Concentration: ~0.1mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC). Purity: Purified by Protein G affinity chromatography from tissue culture supernatant. Immunogen: Bromodeoxyuridine (BSA). Specificity: Recognizes the thymidine analog bromodoxyuridine (BrdU), which can be incorporated into DNA during S-phase of the cell cycle. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1. Magaud, J.P. et al. (1989) Double Immunocytochemical labelling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem.37:1517-1527.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/31/B2850-10H_1.jpg