This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Bromodeoxyuridine (BrdU)
catalog :
B2850-10G
quantity :
200 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
Bu20a
product information
Catalog Number :
B2850-10G
Product wo Prefix :
Bromodeoxyuridine (BrdU)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
200 ug
Clone # USB :
Bu20a
Isotype :
IgG1
Desc1 :
Bromodoxyuridine (BrdU) can be incorporated into DNA during S-phase of the cell cycle. The antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labelling techniques.
Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry (frozen, paraffin). Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:25-1:100; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Alternate Name :
BUdr
Immunogen :
Bromodeoxyuridine (BSA).
Specificity :
Recognizes the thymidine analog bromodoxyuridine (BrdU).
Purity :
Purified by Protein G affinity chromatography from tissue culture supernatant.
Form :
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Concentration :
~1mg/ml
Desc2 :
Product Type: Mab
Isotype: IgG1
Clone No: Bu20a
Host: mouse
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity: Purified by Protein G affinity chromatography.
Immunogen: Bromodeoxyuridine (BSA).
Specificity: Recognizes the thymidine analog bromodoxyuridine (BrdU).
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC IHC
Storage Temperature :
-20°C
Reference :
1. Magaud, J.P. et al. (1989) Double Immunocytochemical labelling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem.37:1517-1527.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/22/B2850-10G_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
service@usbio.net
https://www.usbio.net800-520-3011
headquarters: USA
United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience.
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