Bromodeoxyuridine (BrdU) discontinued | US Biological B2850-10B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU) discontinued

catalog :

B2850-10B

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10E10(Bu20a)

product information

Catalog Number :

B2850-10B

Product wo Prefix :

Bromodeoxyuridine (BrdU) discontinued

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

10E10(Bu20a)

Isotype :

IgG1

Desc1 :

Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues (1,2). BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle (3,4). After pulse-labeling cells or tissues with bromodeoxyuridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure as well as denaturation of double stranded DNA for various immunodetection applications. Applications: Suitable for use in Immunohistochemistry (P), Immunofluorescence (IF-IC) and Flow Cytometry. Other applications not tested. Recommended Dilution: Immunohistochemistry (P): 1:100 Unmasking buffer: Citrate Immunofluorescence (IF-IC): 1:1400 Flow Cytometry: 1:800 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

BrdU conjugated to BSA.

Specificity :

Recognizes human BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, ≤0.02% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 10E10(Bu20a) Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, ≤0.02% sodium azide. Immunogen: BrdU conjugated to BSA. Specificity: Recognizes human BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IC IF

Storage Temperature :

-20°C

Reference :

1. Darzynkiewicz, Z. and Juan, G. (2001) Curr Protoc Cytom Chapter 7, Unit 7.7, 2. Leif, R.C. et al. (2004) Cytometry A 58, 45-52, 3. Staszkiewicz, J. et al. (2009) Biochem Biophys Res Commun 378, 539-44, 4. Rothaeusler, K. and Baumgarth, N. (2007) Curr Protoc Cytom Chapter 7, Unit7.31.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/27/B2850-10B_1.jpg