Bromodeoxyuridine (BrdU) (Biotin) | US Biological B2850-10 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU) (Biotin)

catalog :

B2850-10

quantity :

75 ug

clonality :

monoclonal

host :

mouse

conjugate :

biotin

clone name :

2Q392 (PRB-1)

product information

Catalog Number :

B2850-10

Product wo Prefix :

Bromodeoxyuridine (BrdU) (Biotin)

Host :

mouse

Product Type :

Mab

Conjugate :

Biotin

Category :

Antibodies

Size1 :

75 ug

Clone # USB :

2Q392 (PRB-1)

Isotype :

IgG1

Desc1 :

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:20 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Iodouridine conjugated to ovalbumin.

Specificity :

Recognizes BrdU in ssDNA, free BrdU or BrdU coupled to a protein carrier. Crossreactivity: iodouridine. Does not react with thymidine.

Purity :

Purified immunoglobulin

Form :

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.

Concentration :

~250ug/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 2Q392 (PRB-1) Host: mouse Concentration: ~250ug/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide. Purity: Purified immunoglobulin Immunogen: Iodouridine conjugated to ovalbumin. Specificity: Recognizes BrdU in ssDNA, free BrdU or BrdU coupled to a protein carrier. Crossreactivity: iodouridine. Does not react with thymidine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C