antibody

Bromodeoxyuridine (BrdU) discontinued

B2850-07

500 ul

monoclonal

mouse

nonconjugated

0.N.35

Bromodeoxyuridine (BrdU) discontinued

mouse

Mab

Antibodies

500 ul

0.N.35

IgG1

BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. Applications: Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (formalin/paraffin): 1:500 for 32 minutes at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT, followed by digestion with trypsin at 1mg/ml PBS, 10 minutes at 37ºC . Optimal dilutions to be determined by the researcher. Positive Control: Cultured cells grown in prescence of BrdU or tissues from experimental animals injected with BrdU. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bromodeoxyuridine (BrdU) conjugated to BSA. Cellular Localization: Nuclear

Recognizes Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU conjugated to a protein carrier, as well as free BrdU. Species Crossreactivity: all species

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See B2850-07X.

~0.2mg/ml

Product Type: Mab Isotype: IgG1 Clone No: 0.N.35 Host: mouse Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See B2850-07X. Purity: Purified by Protein G affinity chromatography. Immunogen: Bromodeoxyuridine (BrdU) conjugated to BSA. Cellular Localization: Nuclear Specificity: Recognizes Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU conjugated to a protein carrier, as well as free BrdU. Species Crossreactivity: all species Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF IHC

-20°C

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