Bromodeoxyuridine (BrdU) discontinued | US Biological B2850-03A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU) discontinued

catalog :

B2850-03A

quantity :

1 ml

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

5F214

product information

Catalog Number :

B2850-03A

Product wo Prefix :

Bromodeoxyuridine (BrdU) discontinued

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

1 ml

Clone # USB :

5F214

Isotype :

IgG1

Desc1 :

Applications: Suitable for use in In situ-hybridization, Flow Cytometry and Immunohistochemistry (Frozen and paraffin sections) Recommended Dilution: Immunohistochemistry: 1:10; Incubation Time 1 hr at room temperature. It can be used for: 1. Radioimmunocytochemical detection of circulating levels of BrdU 2 Detection of S-phase cells in tissue sections by immunoperoxidase or immunofluorescence method 3. Detection of S-phase cells in cell suspension 4. Determination of the percentage of profilerating cells by flow cytometry 5. Quantitative determination of the number of various phases of the cell cycle by dual parameter flow-cytometrical analysis Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

BrdU conjugated to BSA

Specificity :

Reacts with BrdU in denatured (single-stranded) DNA). The antibody is crossreactive with iododeoxyuridine. Species Crossreactivity: All species.

Purity :

Ascites

Form :

Supplied as a liquid.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 5F214 Host: mouse Concentration: Not determined Form: Supplied as a liquid. Purity: Ascites Immunogen: BrdU conjugated to BSA Specificity: Reacts with BrdU in denatured (single-stranded) DNA). The antibody is crossreactive with iododeoxyuridine. Species Crossreactivity: All species. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IHC

Storage Temperature :

-20°C

Reference :

Schutte B et al. Effect of Tissue Fixation on Anti-Bromodeoxyuridine Immunohistochemistry. The Journal of Immunohistochemistry and Cytochemistry, Vol 35, 11:1343-1345 (1987) Schutte B et al. Studies with Anti-Bromodeoxyuridine Antibodies: II. Simultaneous Immunocytochemical Detection of Antigen Expression and DNA Synthesis by in Vivo Labeling of Mouse Intestinal Mucosa. The Journal of Histochemistry and Cytochemistry, Vol 35, 3:371-374 (1987) Schutte B et al: Studies with Anti-Bromodeoxyuridine Antibodies: I An improved method for the immunocytochemical detection of BrdfU labeled nuclei using flow cytometry. Cytometry 8:372-376 (1987)