Bromodeoxyuridine (BrdU) | US Biological B2850-03 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU)

catalog :

B2850-03

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

BU5.1

product information

Catalog Number :

B2850-03

Product wo Prefix :

Bromodeoxyuridine (BrdU)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

Bu5.1

Isotype :

IgG2a

Desc1 :

Applications: Suitable for use in Immunofluorescence microscopy. Also suitable for Detection of bromodeoxyuridine incorporation in tissue and cell culture; 3H-thymidine labeling techniques can be avoided. Other applications not tested. Recommended Dilution: Immunofluorescence microscopy: 1:10; Incubation Time 30 min at RT. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

Bromodeoxyuridine

Specificity :

Recognizes bromodesoxyuridine. Does not crossreact with thymidine. It is a valuable tool for detection of S-phase (DNA-synthesizing cells) in cell and tissue culture.

Purity :

Purified by Protein A affinity chromatography.

Form :

Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.

Concentration :

~0.1mg/ml (after reconstitution)

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: Bu5.1 Host: mouse Concentration: ~0.1mg/ml (after reconstitution) Form: Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml. Purity: Purified by Protein A affinity chromatography. Immunogen: Bromodeoxyuridine. Specificity: Recognizes bromodesoxyuridine. Does not crossreact with thymidine. It is a valuable tool for detection of S-phase (DNA-synthesizing cells) in cell and tissue culture. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

Hosihino, T., Nagashima, T., Murovic, J., Levin, E.L., Levin V.A. and Rupp, St.M.: Cell kinetic studies of in situ human brain tumors with bromodeoxyuridine. Cytometrie 6, 627-632 (1985). Jirikowski G.F., Ramalho-Ortigao F., Kesse K.W. and Bloom F.E.: In situ hybridization of semithin Epon sections with BrdU labelled oligonucleotide probes. Histochemistry 94, 187-190 (1990)