Bromodeoxyuridine (BrdU) (FITC) discontinued | US Biological B2850-02D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU) (FITC) discontinued

catalog :

B2850-02D

quantity :

1 ml

clonality :

monoclonal

host :

rat

conjugate :

FITC

clone name :

7E101

product information

Catalog Number :

B2850-02D

Product wo Prefix :

Bromodeoxyuridine (BrdU) (FITC) discontinued

Host :

rat

Product Type :

Mab

Conjugate :

FITC

Category :

Antibodies

Size1 :

1 ml

Clone # USB :

7E101

Isotype :

IgG2a

Desc1 :

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. Applications: Suitable for use in Flow Cytometry, Immunohistology. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat 20ul labels 10e6 cells in 100ul. Immunohistology: Neat 1/20 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

Bromodeoxyuridine (BrdU).

Specificity :

Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU.

Purity :

Purified by Ion Exchange chromatography.

Form :

Supplied as a liquid in PBS, 0.01% sodium azide, before the addition of 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC).

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 7E101 Host: rat Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, 0.01% sodium azide, before the addition of 50% glycerol. Labeled with Fluorescein isothiocyanate (FITC). Purity: Purified by Ion Exchange chromatography. Immunogen: Bromodeoxyuridine (BrdU). Specificity: Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IHC

Storage Temperature :

4°C/-20°C