Bromodeoxyuridine (BrdU) (HRP) discontinued | US Biological B2850-01R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU) (HRP) discontinued

catalog :

B2850-01R

quantity :

500 ug

clonality :

monoclonal

host :

rat

conjugate :

HRP

clone name :

3H579

product information

Catalog Number :

B2850-01R

Product wo Prefix :

Bromodeoxyuridine (BrdU) (HRP) discontinued

Host :

rat

Product Type :

Mab

Conjugate :

HRP

Category :

Antibodies

Size1 :

500 ug

Clone # USB :

3H579

Isotype :

IgG2a

Desc1 :

Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incorporated into DNA during DNA synthesis. Detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. Applications: Suitable for use in ELISA and Immunohistochemistry (paraffin). Other applications not tested. Recommended Dilution: ELISA: 1:250-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Immunogen :

Bromodeoxyuridine (BrdU).

Specificity :

Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. It does not cross react with thymidine but does react weakly with chlorodeoxyuridine.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, pH7.4, 0.01% thiomersal. Labeled with HRP.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 3H579 Host: rat Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH7.4, 0.01% thiomersal. Labeled with HRP. Purity: Purified by Protein G affinity chromatography. Immunogen: Bromodeoxyuridine (BrdU). Specificity: Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. It does not cross react with thymidine but does react weakly with chlorodeoxyuridine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC

Storage Temperature :

-20°C