Bromodeoxyuridine (BrdU) | US Biological B2850-01F product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU)

catalog :

B2850-01F

quantity :

100 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4A9

product information

Catalog Number :

B2850-01F

Product wo Prefix :

Bromodeoxyuridine (BrdU)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ug

Clone # USB :

4A9

Isotype :

IgG1

Desc1 :

The life cycle of a eukaryotic cell consists of various phases, two of which can easily be identified. Firstly, during mitosis (M-phase), in which the cell divides into two identical daughter cells, chromosome condensation and spindle formation are microscopically visible. Secondly, in S-phase the DNA of a cell is replicated, a process that can be detected using biochemical techniques. In between the M- and S-phase two gap phases occur: the G1-phase, the gap between mitosis and the start of DNA replication, and G2-phase, the gap between completion of DNA replication and the onset of mitosis. From G1-phase a cell can leave the cell cycle and enter G0, a ‘quiescent’ phase. Regulation of the cell cycle predominantly occurs at three major control points, which govern the transition from G0 to G1, from G1 to S and from G2 to M-phase. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cyotmetry: 1:50-1:100 Immunohistochemistry (Frozen and paraffin sections): Detection antibody with avidin-biotinylated horseradish peroxidase complex. Optimal dilutions to be determined by researcher. Hybridoma: Hybridization of myeloma cells from SP2/0-Ag14 mice with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunogen :

Bromodeoxyuridine (BrdU)-conjugate to BSA.

Specificity :

Bromodeoxuridine, also when incorporated into nuclear DNA.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 4A9 Host: mouse Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added. Purity: Purified by Protein G affinity chromatography. Immunogen: Bromodeoxyuridine (BrdU)-conjugate to BSA. Specificity: Bromodeoxuridine, also when incorporated into nuclear DNA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IC IHC

Storage Temperature :

-20°C

Reference :

1. Schutte, B., et al., An improved method for the immunocytochemical detection of bromodeoxyuridine labeled nuclei using flow cytometry, Cytometry 8: 372-6 (1987). 2. Tinnemans, M.M., et al., Cytokinetic analysis of lung cancer by in vivo bromodeoxyuridine labelling, Br. J. Cancer 67: 1217-22 (1993). 3. van Engeland, M., et al., Plasma membrane alterations and cytoskeletal changes in apoptosis, Exp. Cell Res. 235: 421-430 (1997). 4. Schutte, B., et al., The effect of the cyclin-dependent kinase inhibitor olomoucine on cell cycle kinetics, Exp. Cell Res. 236: 4-15 (1997).