Bromodeoxyuridine (BrdU) discontinued | US Biological B2850-01E product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU) discontinued

catalog :

B2850-01E

quantity :

1 ml

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

IIB5

product information

Catalog Number :

B2850-01E

Product wo Prefix :

Bromodeoxyuridine (BrdU) discontinued

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

1 ml

Clone # USB :

IIb5

Isotype :

IgG1

Desc1 :

BrdU is a thymidine analogue and when offered to proliferating cells it is incroporated into reduplicating DNA. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen, paraffin): 1:5-1:10 Dilute in 0.15M PBS, 1% BSA, 1% sodium-azide. Requires protease digestion of paraffin sections. Immunocytochemistry: Pulse labeling of dividing cells will allow the immunocytochemical identification of S-phase cells. Optimal dilutions to be determined by the researcher. Positive Control: Immunohistochemistry: Bromodeoxyuridine labeled cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

BrdU (BSA)

Specificity :

Recognizes DNA in which BrdU has been incorporated. Reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. Recognizes BrdU in denaturated (single stranded) DNA. 100% crossreactive with Jodo-Deoxy-Uridine (IrdU).

Purity :

Ascites

Form :

Supplied as a liquid, 0.02% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: IIb5 Host: mouse Concentration: Not determined Form: Supplied as a liquid, 0.02% sodium azide. Purity: Ascites Immunogen: BrdU (BSA) Specificity: Recognizes DNA in which BrdU has been incorporated. Reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. Recognizes BrdU in denaturated (single stranded) DNA. 100% crossreactive with Jodo-Deoxy-Uridine (IrdU). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IC IF IHC

Storage Temperature :

-20°C

Reference :

1. Schutte, B., et al., 1987, J. of Histochemistry and Cytochemistry 35, 371-374. Schutte, B., et al., 1987, J. of Histochemistry and Cytochemistry 35, 1343-1345 2. Schutte, B., et al., 1987, Cytometry 8, 372-376.