Bromodeoxyuridine (BrdU) discontinued | US Biological B2850-01B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bromodeoxyuridine (BrdU) discontinued

catalog :

B2850-01B

quantity :

200 ug

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

3H579

product information

Catalog Number :

B2850-01B

Product wo Prefix :

Bromodeoxyuridine (BrdU) discontinued

Host :

rat

Product Type :

Mab

Category :

Antibodies

Size1 :

200 ug

Clone # USB :

3H579

Isotype :

IgG2a

Desc1 :

BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:25-1:200. Use 20ul to label 10e6 cells in 100ul Immunohistochemistry (Paraffin): 1:25-1:200 Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1904-49H IgG, F(ab’)2, H&L X-Adsorbed (HRP) RbxRt I1904-49U IgG, F(ab’)2, H&L X-Adsorbed (FITC) RbxRt Recommended Negative Controls: I1904-83M1: IgG2a, Rat Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen :

Bromodeoxyuridine (BrdU)

Specificity :

Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Does not crossreact with thymidine but does react weakly with chlorodeoxyuridine.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.09% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: 3H579 Host: rat Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, 0.09% sodium azide. Purity: Purified by Protein G affinity chromatography. Immunogen: Bromodeoxyuridine (BrdU) Specificity: Recognizes BrdU incorporated into single stranded DNA, attached to a protein carrier and free BrdU. Does not crossreact with thymidine but does react weakly with chlorodeoxyuridine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IHC

Storage Temperature :

-20°C

Reference :

1. Vanderlaan, M. & Thomas, C.B. (1985) Characterization of monoclonal antibodies to bromodeoxyuridine. Cytometry. 6: 501-505. 2. Ghiringelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation. J. Exp. Med. 202: 919-929. 3. Dolbeare, F. (1995) Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, histochemical methods and cell kinetics. Histochem. J. 27: 339-369.