antibody

Bone Morphogenetic Protein 2 (BMP 2, BMP-2, BMP2, BMP 2A, BMP2A, BMP-2A)

B2553-07G

50 ug

polyclonal

domestic rabbit

nonconjugated

Bone Morphogenetic Protein 2 (BMP 2, BMP-2, BMP2, BMP 2A, BMP2A, BMP-2A)

rabbit

Pab

Antibodies

50 ug

IgG

BMP-2 is a member of the Transforming Growth Factor beta (TGF-B) superfamily and one of a growing number of osteogenic proteins shown to induce bone and cartilage formation and to play an important role in developmental processes, including cell proliferation, differentiation, apoptosis and morphogenesis. BMPs act through binding with a receptor complex consisting of type I and type II serine/threonine kinases, resulting ultimately in the activation of the Smad protein and mitogen-activated protein kinase (MAPK) signaling pathways. Several antagonist proteins, including, noggin, chordin, gremlin and follistatin, are responsible for modulating the signaling effects of BMPs, through the binding and blocking of receptor ligands, thereby preventing activation. The realization that BMP-2 was involved in the stimulation of bone formation emerged from research in spinal surgery, following the discovery that the healing of bones was directed by proteins contained within the bone matrix itself. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-5ug/ml Western Blot: 0.1-1ug/ml, reducing or non-reducing conditions Immunohistochemistry (paraffin): 0.25ug/ml, requires antigen retrieval using heat treatment prior to staining of paraffin sections. It is recommended to use sodium citrate buffer, pH 6.0 for this purpose. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Bone Morphogenetic Protein 2 Preproprotein

Recombinant protein corresponding to human BMP-2.

Recognizes human BMP-2.

Purified

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

~1mg/ml

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Purity: Purified Immunogen: Recombinant protein corresponding to human BMP-2. Specificity: Recognizes human BMP-2. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IF IHC WB

-20°C

1. Balemans, W. and Van Hul, W. (2002) Extracellular regulation of BMP signaling in vertebrates: a cocktail of modulators. Dev. Biol. 250: 231-250. 2. Sebald, W. et al. (2004) Molecular recognition in bone morphogenetic protein (BMP)/receptor interaction. Biol. Chem. 385: 697-710. 3. Keller, S. et al. (2004) Molecular recognition of BMP-2 and BMP receptor IA. Nature Structural and Molecular Biology. 11: 481-488. 4. Kirsch, T. et al. (2000) BMP-2 antagonists emerge from alterations in the low-affinity binding epitope for receptor BMPR-II. EMBO J. 19: 3314-3324. 5. Kimura, N. et al. (2000) BMP2-induced apoptosis is mediated by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6. J. Biol. Chem. 275: 17647-17652.

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