Blk | US Biological B1900 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Blk

catalog :

B1900

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

B1900

Product wo Prefix :

Blk

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development (1,2). Activation of B cells by various ligands is accompanied by activation of Blk (3). It has been suggested that Blk is involved in the control of B cell differentiation and proliferation (4,5). Except expressed in B cell, Blk transcripts were also detected in human thymocytes but not in mature T cells, implicating that Blk may play an important role in thymopoiesis (6). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to residues surrounding the C-terminus of human Blk (KLH).

Specificity :

Recognizes total levels of endogenous human Blk.

Purity :

Purified by Protein A and immunoaffinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding the C-terminus of human Blk (KLH). Specificity: Recognizes total levels of endogenous human Blk. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Dymecki, S.M., et al., Science 247: 332-336 (1990). 2. Wasserman, R., et al., J. Immunol. 155: 644-651 (1995). 3. Sefton, B.M., et al., Curr. Opin. Immunol. 6: 372-379 (1994). 4. Malek, S.N., et al., Proc. Natl. Acad. Sci. USA 95: 7351-7356 (1998). 5. Yao, X.R., et al., Proc. Natl. Acad. Sci. USA 90: 7946-7950 (1993). 6. Islam, K.B., et al., J. Immunol. 154: 1265-1272 (1995).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/17/B1900_1.jpg