BiP | US Biological B1770 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

BiP

catalog :

B1770

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

7H9(C50B12)

reactivity :

human

product information

Catalog Number :

B1770

Product wo Prefix :

BiP

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H9(C50B12)

Isotype :

IgG

Desc1 :

Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells. It was also found to be induced at the protein level by glucose starvation. When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists them to refold properly. Applications: Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin):1:200 (Antigen retrieval: Citrate/TBST-5%NGS) Immunohistochemistry (Frozen): 1:200 (10% neutral buffered formalin) Flow Cytometry: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo

Immunogen :

Synthetic peptide corresponding to residues surrounding Gly584 of human BiP.

Specificity :

Recognizes endogenous levels of total human BiP protein at ~78kD. Species Crossreactivity: mouse

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H9(C50B12) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Gly584 of human BiP. Specificity: Recognizes endogenous levels of total human BiP protein at ~78kD. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IHC WB

Storage Temperature :

-20°C

Reference :

US Biological reference: 1. Berthe A., et al. Protein N-glycosylation alteration and glycolysis inhibition both contribute to the antiproliferative action of 2-deoxyglucose in breast cancer cells. 2018. Breast Cancer Res Treat. https://doi.org/10.1007/s10549-018-4874-z. 1. Wabl, M. and Steinberg, C. (1982) Proc. Natl. Acad. Sci. USA 79, 6976-6978. 2. Haas, I.G. and Wabl, M. Nature 306, 387-389. 3. Munro, S. and Pelham, H.R. (1986) Cell 46, 291-300. 4. Kohno, K. et al. (1993) Mol. Cell Biol. 13, 877-890.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/39/B1770_1.jpg

Picture4 Text :

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using B1770.