BID (BH3 Interacting Domain Death Agonist) | US Biological B1095-05 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

BID (BH3 Interacting Domain Death Agonist)

catalog :

B1095-05

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

B1095-05

Product wo Prefix :

BID (BH3 Interacting Domain Death Agonist)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

The BH3 domain-only protein BID, a death agonist member of the Bcl-2/Bcl-xL family, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved BID translocates to mitochondria and induces cytochrome c release and mitochondrial damage. Thus, BID relays an apoptotic signal from the cell surface to mitochondria. However, the precise molecular mechanism for the translocation of the cleaved BID and for the subsequent release of cytochrome c during apoptosis is unclear. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to residues surrounding the cleavage site of human BID.

Specificity :

Detects endogenous levels of both the full length (22kD) and cleaved large fragment of human BID (15kD).

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding the cleavage site of human BID. Specificity: Detects endogenous levels of both the full length (22kD) and cleaved large fragment of human BID (15kD). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Wang, K. et al. (1996) Genes Dev. 10, 2859–2869. 2. Luo, X. et al. (1998) Cell 94, 481–490. 3. Li, H. et al. (1998) Cell 94, 491–501. 4. Gross, A. et al. (1999) J. Biol. Chem. 274, 1156–1163. 5. Yin, X.M. et al. (1999) Nature 400, 886–891. 6. Mandic, A. et al. (2002) Mol. Cell. Biol. 22(9), 3003–3013.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/31/B1095-05_1.jpg