antibody

Bim (Bod, Bcl2l11, BCL2-like 11 Apoptosis Facilitator, Bcl2 Interacting Mediator of Cell Death)

B1090-01D

100 ul

polyclonal

domestic rabbit

nonconjugated

Bim (Bod, Bcl2l11, BCL2-like 11 Apoptosis Facilitator, Bcl2 Interacting Mediator of Cell Death)

rabbit

Pab

Antibodies

100 ul

IgG

Bim/Bod is a pro-apoptotic protein belonging to the -BH3-only+ group of Bcl-2 family members (that includes Bad, Bid, Bik, Hrk and Noxa) containing a BH3 domain but lacking other conserved domains, BH1 or BH2 (1,2). Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1 and BHRF-1 (1,2). Particular functions for Bim have been described in the regulation of apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated (3-6). Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL and BimS (1). The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The other isoforms, BimEL and BimL, may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7). Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8,9). Environmental stress triggers Bim phosphorylation by JNK, resulting in dissociation with the dynein complex and increased apoptotic activity. Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo Rt

Synthetic peptide corresponding to residues near the N-terminus of human Bim. Species Sequence Homology: monkey and porcine

Recognizes endogenous levels of total human Bim (EL, L and S isoforms) at ~23, 15 and 12kD respectively. Species Crossreactivity: mouse, rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues near the N-terminus of human Bim. Species Sequence Homology: monkey and porcine Specificity: Recognizes endogenous levels of total human Bim (EL, L and S isoforms) at ~23, 15 and 12kD respectively. Species Crossreactivity: mouse, rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. O’Connor, L. et al. (1998) EMBO J. 17:384-395. 2. Hsu, S.Y. et al. (1998) Mol. Endocrinol. 12:1432-1440. 3. Bouillet, P. et al. (2002) Nature 415:922-926. 4. Whitfield, J. et al. (2001) Neuron 29:629-643. 5. Dijkers, P.F. et al. (2000) Curr. Biol. 10:1201-1204. 6. Ley, R. et al. (2003) J. Cell Biol. 278:18811-18816. 7. Puthalakath, H. et al. (1999) Mol. Cell 3:287-296. 8. Lei, K. and Davis, R.J. (2003) Proc. Natl. Acad. Sci.USA 100:2432-2437. 9. Putcha, G.V. et al. (2003) Neuron 28:899-914.

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