antibody

Bcl 2, phosphorylated (Ser70) (B Cell Leukemia 2)

B0807-04A

100 ul

monoclonal

domestic rabbit

nonconjugated

phosphorylated

7A4(5H2)

Bcl 2, phosphorylated (Ser70) (B Cell Leukemia 2)

rabbit

Mab

Phosphorylated

Antibodies

100 ul

7A4(5H2)

IgG

Bcl 2 has the ability to exert a survival function in response to a wide range of apoptotic stimuli by blocking mitochondrial release of cytochrome c (1). It has been implicated in modulating mitochondrial Ca2+ homeostasis and proton flux (2). Several phosphorylation sites have been identified within the Bcl 2 loop domain, including serine 70 (3). It has been suggested that phosphorylation at Ser70 may involve an ASK1/MKK7/JNK1 pathway and may be a marker for mitotic events (4,5). Interleukin 3 and JNK induced Bcl 2 phosphorylation at Ser70 may be required for its enhanced antiapoptotic functions (6). Applications: Suitable for use in ELISA, Immunofluorescence, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Western blot analysis of extracts from Jurkat cells, untreated or treated with paclitaxel (1uM, overnight) and with or without λphosphatase, using phospho-Bcl-2 (Ser70) Rabbit mAb (upper) or Bcl-2 (lower). Flow Cytometry: 1:25. Flow cytometric analysis of Jurkat cells, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb versus propidium iodide (DNA content). Immunofluorescence (Immunocytochemistry): 1:200. Immunofluorescent analysis of MCF-7 cells with or without treatment of paclitaxel (1uM, overnight), using phospho-Bcl-2 (Ser70) Rabbit mAb. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Synthetic phosphopeptide corresponding to residues surrounding Ser70 of human Bcl 2.

Recognizes endogenous levels of human Ser70 phosphorylated Bcl 2. It does not crossreact with non-phosphorylated Bcl 2 at endogenous levels or with other Bcl 2 family members.

Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 7A4(5H2) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified by Protein A affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser70 of human Bcl 2. Specificity: Recognizes endogenous levels of human Ser70 phosphorylated Bcl 2. It does not crossreact with non-phosphorylated Bcl 2 at endogenous levels or with other Bcl 2 family members. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF WB

-20°C

1. Murphy, K.M. et al. (2000) Cell Death Differ. 7, 102–111. 2. Zhu, L. et al. (1999) J. Biol. Chem. 274, 33267–33273. 3. Fadeel, B. (1999) Leukemia 13, 719–728. 4. Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469–8478. 5. Ling, Y.H. et al. (1998) J. Biol. Chem. 273, 18984–18991. 6. Deng, X. et al. (2001) J. Biol. Chem. 276, 23681–23688.

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