BAP31 (B Cell Receptor-Associated Protein 31, BCR-Associated Protein, p28, CDM Protein, 6C6-AG Tumor-associated Antigen, DXS1357E) | US Biological B0077-03 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

BAP31 (B Cell Receptor-Associated Protein 31, BCR-Associated Protein, p28, CDM Protein, 6C6-AG Tumor-associated Antigen, DXS1357E)

catalog :

B0077-03

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

B0077-03

Product wo Prefix :

BAP31 (B Cell Receptor-Associated Protein 31, BCR-Associated Protein, p28, CDM Protein, 6C6-AG Tumor-associated Antigen, DXS1357E)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

BAP31 (B-cell receptor-association protein 31) is a transmembrane protein associated with the endplasmic reticulum (ER) and ER-Golgi intermediates and has been implicated in protein trafficking and apoptosis (1,2). During apoptosis Bap31 is cleaved by caspase-8 at two carboxy-terminal sites which can then direct apoptotic signals between the ER and mitochondria (2-4). Association of BAP31 with the anti-apoptotic proteins Bcl-2 or Bcl-xL could function to regulate this ER-mitochondrial pathway (2,5). Several studies have shown that BAP31 can control the trafficking of select proteins between the ER and Golgi apparatus and can affect the transport of proteins to the cell surface (6-10). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to a region surrounding Arg71 of BAP31.

Specificity :

Detects endogenous levels of total human BAP31 protein. Species Crossreactivity: mouse, monkey and rat

Purity :

Purified by peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to a region surrounding Arg71 of BAP31. Specificity: Detects endogenous levels of total human BAP31 protein. Species Crossreactivity: mouse, monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

(1) Adachi, T. et al. (1996) EMBO J 15, 1534–41. (2) Ng, F.W. et al. (1997) J Cell Biol 139, 327–38. (3) Breckenridge, D.G. et al. (2003) J Cell Biol 160, 1115–27. (4) Chandra, D. et al. (2004) Mol Cell Biol 24, 6592–607. (5) Ng, F.W. and Shore, G.C. (1998) J Biol Chem 273, 3140–3. (6) Annaert, W.G. et al. (1997) J Cell Biol 139, 1397–410. (7) Lambert, G. et al. (2001) J Biol Chem 276, 20340–5. (8) Schamel, W.W. et al. (2003) Proc Natl Acad Sci USA 100, 9861–6. (9) Paquet, M.E. et al. (2004) J Immunol 172, 7548–55. (10) Szczesna-Skorupa, E. and Kemper, B. (2006) J Biol Chem 281, 4142–8.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/15/B0077-03_1.jpg