antibody

Bad, phosphorylated (Ser136) (Bcl2 Antagonist of Cell Death)

B0003-58

200 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Bad, phosphorylated (Ser136) (Bcl2 Antagonist of Cell Death)

rabbit

Pab

Phosphorylated

Antibodies

200 ul

IgG

Bad is a proapoptotic member of the Bcl-2 family that can displace Bax from binding to Bcl-2 and Bcl-xL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-2 and Bcl-xL. Akt has been shown to promote cell survival via its ability to phosphorylate Bad at Ser136. Ser112 has been shown to be the substrate in vivo and in vitro of p90RSK and mitochondria-anchored PKA. Phosphorylation of Ser155 by PKA plays a critical role in blocking the dimerization of Bad to Bcl-xL because of its position in the BH3 domain. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500. Diluted antibody in TBS, 5% BSA, 0.1% Tween-20. Incubate at 4°C overnight with gentle shaking. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Mo

Synthetic phosphopeptide corresponding to residues around Ser136 of mouse Bad. Species sequence homology: human and rat (100%)

Recognizes transfected levels of Bad only when phosphorylated at Ser136. Does not detect non-phosphorylated Bad or Bad phosphorylated at other sites. Does not detect related family members. Species crossreactivity: mouse.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: mouse Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues around Ser136 of mouse Bad. Species sequence homology: human and rat (100%) Specificity: Recognizes transfected levels of Bad only when phosphorylated at Ser136. Does not detect non-phosphorylated Bad or Bad phosphorylated at other sites. Does not detect related family members. Species crossreactivity: mouse. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Yang, E., et al. (1995) Cell 80: 285-291. 2. Zha, J., et al. (1996) Cell 87: 619-628. 3. Datta, S.R., et al. (1997) Cell 91: 231-241. 4. Mizushima, S. and Nagata, S. (1990) Nucleic Acids Res. 18: 5322. 5. Peso, L., et al. (1997) Science 278: 687-689. 6. Bonni, A., et al. (1999) Science 286: 1358-1362. 7. Tan, Y., et al. (1999) J. Biol. Chem. 274: 34,859-34,867. 8. Harada, H., et al. (1999) Mol. Cell 3: 413-422. 9. Tan, Y., et al. (2000) J. Biol. Chem. 275: 25,865-25,869. 10. Lizcano, J. (2000) Biochem. J. 349: 547-557. 11. Datta, S., et al. (2000) Mol. Cell 6: 41-51. 12. Jamieson, C.A. and Yamamoto, K.R. (2000) Proc. Natl. Acad. Sci. USA 97: 7319-7324. 13. Laine, J., et al. (2000) Mol. Cell 6: 395-407. 14. Tan, Y., et al. (1999) J. Biol. Chem. 274: 34,859-34,867.

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