antibody

Bad, phosphorylated (Ser112) (Bcl2 Antagonist of Cell Death)

B0003-57

100 ul

monoclonal

mouse

nonconjugated

phosphorylated

3A37(7E11)

Bad, phosphorylated (Ser112) (Bcl2 Antagonist of Cell Death)

mouse

Mab

Phosphorylated

Antibodies

100 ul

3A37(7E11)

IgG1

Bad is a proapoptotic member of the Bcl-2 family that can displace Bax from binding to Bcl-2 and Bcl-XL, resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-2 and Bcl-XL. Akt has been shown to promote cell survival via its ability to phosphorylate Bad at Ser136. Ser112 has been shown to be the substrate in vivo and in vitro of p90RSK and mitochondria-anchored PKA. Phosphorylation of Ser155 by PKA plays a critical role in blocking the dimerization of Bad to Bcl-XL because of its position in the BH3 domain. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:2000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Ser112 of mouse Bad.

Recognizes endogenous levels of total Bad only when phosphorylated at Ser112. Does not detect Bad phosphorylated at other sites. Nor does it detect related family members. Species Crossreactivity: Human, mouse, monkey and rat. Other species have not been tested.

Ascites

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG1 Clone No: 3A37(7E11) Host: mouse Source: mouse Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser112 of mouse Bad. Specificity: Recognizes endogenous levels of total Bad only when phosphorylated at Ser112. Does not detect Bad phosphorylated at other sites. Nor does it detect related family members. Species Crossreactivity: Human, mouse, monkey and rat. Other species have not been tested. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

1. Yang, E. et al. (1995) Cell 80, 285–291.2. Zha, J. et al. (1996) Cell 87, 619–628.3. Datta, S.R. et al. (1997) Cell 91, 231–241.4. Mizushima, S. and Nagata, S. (1990) Nucleic Acids Res. 18, 5322.5. Peso, L. et al. (1997) Science 278, 687–689.6. Bonni, A. et al. (1999) Science 286, 1358–1362.7. Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859–34867.8. Harada, H. et al. (1999) Mol. Cell 3, 413–422.9. Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865–25869.10. Lizcano, J. (2000) Biochem. J. 349, 547–557.11. Datta, S. et al. (2000) Mol. Cell 6, 41–51.12. Avota, E. et al. (2001) Nat. Med. 7(6), 725–731.13. Bieberich, E. et al. (2001) J. Biol. Chem. 276(48), 44396–44404.

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