Bad, phosphorylated (Ser136) | US Biological B0003-51C1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Bad, phosphorylated (Ser136)

catalog :

B0003-51C1

quantity :

200 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

8A6(185D10)

reactivity :

human

product information

Catalog Number :

B0003-51C1

Product wo Prefix :

Bad, phosphorylated (Ser136)

Host :

rabbit

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

200 ul

Clone # USB :

8A6(185D10)

Isotype :

IgG

Desc1 :

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 protein to prevent an association between Bad and Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Mk Mo

Immunogen :

Synthetic phosphopeptide corresponding to residues surrounding Ser136 of mouse Bad (KLH). Species Sequence Homology: Human.

Specificity :

Recognizes transfected levels of mouse Bad protein only when phosphorylated at Ser136. Species Crossreactivity: Monkey.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 8A6(185D10) Host: rabbit Source: mouse Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser136 of mouse Bad (KLH). Species Sequence Homology: Human. Specificity: Recognizes transfected levels of mouse Bad protein only when phosphorylated at Ser136. Species Crossreactivity: Monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Yang, E., et al., Cell 80: 285–291 (1995). 2. Zha, J., et al., Cell 87: 619–628 (1996). 3. Datta, S.R., et al., Cell 91: 231–241 (1997). 4. Peso, L., et al., Science 278: 687–689 (1997). 5. Bonni, A., et al., Science 286: 1358–1362 (1999). 6. Tan, Y., et al., J. Biol. Chem. 274: 34,859–34,867 (1999). 7. Harada, H., et al., Mol. Cell 3: 413–422 (1999). 8. Tan, Y., et al., J. Biol. Chem. 275: 25,865–25,869 (2000). 9. Lizcano, J., et al., Biochem. J. 349: 547–557 (2000). 10. Datta, S., et al., Mol. Cell 6: 41–51 (2000).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/29/B0003-51C1_1.jpg