Atrophin-1 Interacting Protein 4 (AIF4, ITCH, E3 Ubiquitin-protein Ligase Itchy Homolog, AIP4, NAPP1, NFE2-associated Polypeptide 1, NAPP1) | US Biological A4156-17D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Atrophin-1 Interacting Protein 4 (AIF4, ITCH, E3 Ubiquitin-protein Ligase Itchy Homolog, AIP4, NAPP1, NFE2-associated Polypeptide 1, NAPP1)

catalog :

A4156-17D

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

product information

Catalog Number :

A4156-17D

Product wo Prefix :

Atrophin-1 Interacting Protein 4 (AIF4, ITCH, E3 Ubiquitin-protein Ligase Itchy Homolog, AIP4, NAPP1, NFE2-associated Polypeptide 1, NAPP1)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

ITCH is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and has been shown to interact via its WW domains with DRPLA, NFE2 and CBLC. Other potential interaction partners include NOTCH1, OCLN, JUN and JUNB. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.03-0.1ug/ml, observed in human brain, frontal cortex, lysates on ~100kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Ca Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to C-EIKSHDLKPNGGN, from human ITCH, at the internal region of the protein (NP_113671.3).

Specificity :

Recognizes human ITCH. Species sequence homology: Canine, mouse and rat.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to C-EIKSHDLKPNGGN, from human ITCH, at the internal region of the protein (NP_113671.3). Specificity: Recognizes human ITCH. Species sequence homology: Canine, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Di Marcotullio L, Ferretti E, Greco A, De Smaele E, Po A, Sico MA, Alimandi M, Giannini G, Maroder M, Screpanti I, Gulino A. Numb is a suppressor of Hedgehog signalling and targets Gli1 for Itch-dependent ubiquitination. Nat Cell Biol. 2006 Dec;8(12):1415-23. Epub 2006 Nov 19.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/13/A4156-17D_1.jpg