Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1) | US Biological A3577 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1)

catalog :

A3577

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

3A14(6-24)

reactivity :

human

product information

Catalog Number :

A3577

Product wo Prefix :

Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1)

Host :

mouse

Product Type :

Mab

Antigen Modification :

Phosphorylated

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

3A14(6-24)

Isotype :

IgG1

Desc1 :

Beta-arrestin 1 is a member of the arrestin family, which has been implicated in mediating the desensitization and internalization of G protein-coupled receptors (GPCR). Beta-arrestin 1 is constitutively phosphorylated by Erk1/2 at serine 412 of the C-terminus and localized in the cytosol. When recruited to the plasma membrane after agonist stimulation of the beta2-adrenergic receptors, beta-arrestin 1 is rapidly dephosphorylated. This dephosphorylation is an essential step of beta-arrestin 1-mediated receptor endocytosis, but it is not required for receptor desensitization. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:100. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Ser412 of human beta-arrestin 1.

Specificity :

Recognizes endogenous human beta-arrestin 1 only when phosphorylated at Ser412. Does not crossreact with beta-arrestin 2. Species Crossreactivity: mouse, rat and monkey

Purity :

Ascites

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: 3A14(6-24) Host: mouse Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic peptide corresponding to residues surrounding Ser412 of human beta-arrestin 1. Specificity: Recognizes endogenous human beta-arrestin 1 only when phosphorylated at Ser412. Does not crossreact with beta-arrestin 2. Species Crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IP WB

Storage Temperature :

-20°C

Reference :

1. Lefkowitz, R.J. (1998) J. Biol. Chem. 273, 18677–18680. 2. Lin, F. et al. (1999) J. Biol. Chem. 274, 15971–15974. 3. Lin, F. et al. (1997) J. Biol. Chem. 272, 31051–31057.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/21/A3577_1.jpg