antibody

Arrestin 1,2 beta (ARRB1, ARB1, ARR1, ARRB2, ARB2, ARR2)

A3577-01H

100 ul

monoclonal

domestic rabbit

nonconjugated

10E5(D24H9)

Arrestin 1,2 beta (ARRB1, ARB1, ARR1, ARRB2, ARB2, ARR2)

rabbit

Mab

Antibodies

100 ul

10E5(D24H9)

IgG

Arrestin proteins function as negative regulators of G protein coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylat-ed GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling (1). Four distinct mam-malian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (or X-arrestin) are localized to retinal rods and cones, respectively. Arrestin 2 (also known as Beta-arrestin 1) and arrestin 3 (or Beta-arrestin 2) are ubiqui-tously expressed and bind to most GPCRs (2). Beta-arrestin proteins function as adapters and scaffold proteins and play important roles in other processes, such as recruiting c-Src family proteins to GPCRs in ERK activation pathways (3,4). Beta arrestins are also involved in some receptor tyrosine kinase signaling pathways (5-8). Additional evidence suggests that Beta-arrestin proteins translocate to the nucleus and help regulate transcription by binding transcriptional cofactors (9,10). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Recombinant protein corresponding to the C-terminal region of human beta-arrestin 1.

Recongnizes endogenous levels of human total beta-arrestin 1 and beta-arrestin 2 proteins. Species Crossreactivity: Rat, mouse and monkey

Purified by Protein A affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10E5(D24H9) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified by Protein A affinity chromatography. Immunogen: Recombinant protein corresponding to the C-terminal region of human beta-arrestin 1. Specificity: Recongnizes endogenous levels of human total beta-arrestin 1 and beta-arrestin 2 proteins. Species Crossreactivity: Rat, mouse and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

IP WB

-20°C

1. Shenoy, S.K. and Lefkowitz, R.J. (2005) Sci STKE 2005, cm10, 2. Lefkowitz, R.J. and Shenoy, S.K. (2005) Science 308, 512-7, 3. Luttrell, L.M. et al. (1999) Science 283, 655-61, 4. Luttrell, L.M. et al. (1999) Curr Opin Cell Biol 11, 177-83, 5. Luttrell, L.M. and Lefkowitz, R.J. (2002) J Cell Sci 115, 455-65, 6. Waters, C. et al. (2004) Semin Cell Dev Biol 15, 309-23, 7. Lefkowitz, R.J. and Whalen, E.J. (2004) Curr Opin Cell Biol 16, 162-8, 8 Waters, C.M. et al. (2005) Cell Signal 17, 263-77, 9. Kang, J. et al. (2005) Cell 123, 833-47, 10. Ma, L. and Pei, G. (2007) J Cell Sci 120, 213-8.

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