Apg12 (Autophagy-related 12, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12) | US Biological A2298-76D product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Apg12 (Autophagy-related 12, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)

catalog :

A2298-76D

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

A2298-76D

Product wo Prefix :

Apg12 (Autophagy-related 12, Atg12, Apg12L, APG12-like, Atg12L, Autophagy-related protein 12)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Autophagy is a catabolic process for the autophagosomic- lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegenerative diseases, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Apg12 is covalently bound to Apg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Apg7 and the E2-like enzyme Apg10 (7,8). Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100 Western Blot: 1:1000 Immunoprecipitation: 1:25 Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to residues near the N-terminus of human Atg12 (KLH).

Specificity :

Detects endogenous levels of total free at 16kD and Atg5 bound Atg12 protein at 53kD.

Purity :

Purified by peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues near the N-terminus of human Atg12 (KLH). Specificity: Detects endogenous levels of total free at 16kD and Atg5 bound Atg12 protein at 53kD. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IF IP WB

Storage Temperature :

-20°C

Reference :

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1,11–21. 2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ12 Suppl 2, 1509–18. 3. Levine, B. and Yuan, J. (2005) J Clin Invest 115,2679–88. 4. Mizushima, N. et al. (1998) J Biol Chem 273,33889–92. 5. Mizushima, N. et al. (1998) Nature 395, 395–8. 6. Suzuki, K. et al. (2001) EMBO J 20, 5971–81. 7. Tanida, I. et al. (1999) Mol Biol Cell 10, 1367–79. 8. Shintani, T. et al. (1999) EMBO J 18, 5234–41.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/22/A2298-76D_1.jpg